• br Discussion Since the derivation of HESCs multiple

  2018-10-22

  

  Discussion Since the derivation of HESCs multiple differentiation protocols towards specific endodermal derivatives have been established (D\'Amour et al., 2006; Jiang et al., 2007a, 2007b; Shim et al., 2007; Roelandt et al., 2010; Si-Tayeb et al., 2010; Van Vranken et al., 2007; Wang et al., 20

• A limitation of the study

  2018-10-22

  

  A limitation of the study is the potential bias of the IV approach of administration. The proximity of the tail vein to the anal sphincter may suggest that MSCs could be trapped during their systemic course. Ours was an initial study and a longer time course of recovery is underway with different do

• Concomitant with its suppressive effect on blast colony form

  2018-10-22

  

  Concomitant with its suppressive effect on blast colony formation, the inclusion of WNT3A, or the WNT agonist BIO, in methylcellulose cultures promoted the emergence of colonies we termed “mesospheres” (Figure 7H). These colonies were capable of osteogenic, adipogenic, and smooth muscle differentiat

• br Results br Discussion Despite the widespread

  2018-10-22

  

  Results Discussion Despite the widespread clinical use of hMSCs, there are fundamental gaps in our knowledge of basic MSC biology. In contrast to HSCs, the molecular mechanisms that maintain MSCs in their undifferentiated state or determine their differentiation pathways are still poorly under

• Tumor microenvironment plays critical roles in regulating tu

  2018-10-22

  

  Tumor microenvironment plays critical roles in regulating tumor evolution, growth and metastasis (Allen and Louise Jones, 2010; Kenny et al., 2007). Cancer ae3 interact with their extracellular matrix (ECM) during proliferation and migration. Fibronectin is an important ECM glycoprotein with severa

• When spinal cords were kept in vitro for h

  2018-10-20

  

  When spinal cords were kept in vitro for 24h, we observed a novel phenomenon, namely centrifugal mobilization of ependymal stem cells which formed a migratory chain analogous to the NU 7441 Supplier RMS (Lois et al., 1996; Wichterle et al., 1997; Tanvig et al., 2009) as they moved away from the cent

• order BI6727 br Materials and methods br Results br Discussi

  2018-10-20

  

  Materials and methods Results Discussion Strategies aiming at controlled divergence of ASCs to specific differentiated cell types essentially rely on prolonged culture in the presence of specific sets of growth factors and/or matrix proteins (Zuk et al., 2001). Specific culture conditions w

• Myoseverin Dibromo chloropropane DBCP is a banned

  2018-10-20

  

  1,2-Dibromo-3-chloropropane (DBCP) is a banned nematicide that has been shown to cause male infertility (Amann and Berndtson, 1986; Bjorge et al., 1995; Holme et al., 1991; Potashnik and Porath, 1995; Slutsky et al., 1999; Whorton et al., 1979). In exposed workers, DBCP is gonadotoxic resulting in a

• Immunocytochemistry Cells were fixed at room temperature wit

  2018-10-20

  

  Immunocytochemistry. Cells were fixed at room temperature with 4% paraformaldehyde for 10min. Non-specific proteins were blocked by ultra V block (Thermo). The cells were then treated with primary ampa overnight at 4°C. Primary antibodies were against TRA-1-60 (1:500, MA1-023, Thermo Fisher), OCT4

• Our results are in line with previous findings showing that

  2018-10-20

  

  Our results are in line with previous findings showing that cellular senescence negatively correlates with reprogramming efficiency (Banito et al., 2009; Mali et al., 2008; Qin et al., 2007; Zhao and Daley, 2008; Zhao et al., 2008). The expression of p21 is controlled by p53 during the replicative

• Progression of maladaptive LV remodeling increased wall stre

  2018-10-20

  

  Progression of maladaptive LV remodeling, increased wall stress and transition to heart failure are associated with an enhanced cardiac production of natriuretic peptides (Hohl et al. 2013; van Veldhuisen et al. 2013). TAC led to a marked five- to twenty-fold increase of myocardial BNP and ANP expre

• br Materials and methods br Acknowledgments

  2018-10-20

  

  Materials and methods Acknowledgments This study was supported by postdoctoral research station of Central South University and funded by China Postdoctoral Science Foundation (2014M560650 to S.Z.), the National Natural Science Foundation of China (grant number 31401248 to S.Z.), Natural Scien

• Background green fluorescence was detected in the lentiviral

  2018-10-20

  

  Background green fluorescence was detected in the lentiviral reprogrammed MML-6838-CL2 iPSC line and a control episomal (non-GFP) reprogrammed iPSC, indicating silencing (Fig. 1B). Pluripotency was confirmed by detecting expression of SOX2, OCT4, SSEA4, TRA1-61 and TRA1-80 pluripotency-associated ma

• matrix metalloproteinase The in vitro conditions for RA indu

  2018-10-20

  

  The in vitro conditions for RA-induced FP induction required high concentrations of RA, pointing possibly to an epiphenomenon that jump starts a self-enhancing activator/inhibitor signaling system, leading to localized FP formation. Both the FoxA2 gene, a transcription factor required for SHH expres

• A key element of the

  2018-10-20

  

  A key element of the culture conditions is the balanced combination of growth factors and signaling molecules (e.g., EGF, bFGF, IGF, insulin, transferrin, and Rock inhibitor), which apparently generates an artificial androgen-independent PESC microenvironment that promotes the self-renewal and maint

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